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Publications
1) Partridge et al. (1999) Competition between the signal sequence and a 3`UTR localisation signal during redirection of beta-globin mRNA to endoplasmic reticulum: implications for biotechnology. Cytotechnology 30: 37-47. Read paper
2) Corporate profile: UniTargetingResearch enhances protein production. Genetic Engineering News, 2004, Vol. 24, No. 2. Read paper
3) Stern, B and Pryme, I.F. (2005) Improved protein production from mammalian cells. BIOforum Europe 6: 38-40. Read paper
4) Knappskog et al. (2007) The level of synthesis and secretion of Gaussia princeps luciferase in transfected CHO cells is heavily dependent on the choice of signal peptide. J. Biotechnol. 128: 705-715. Read paper
5) Stern, B., Gjerdrum, C., Knappskog, S., Minsaas, L., Nyland, S., Olsen, L.C., Olsen, L., Oveland, E., Ravneberg, H., Tröße, C., Tveit, A., Vollsund, E., Hesketh, J.E., Tauler, A. and Pryme I.F. UTRtechTM : Exploiting mRNA targeting to increase protein secretion from mammalian cells, In: Cell Technology for Cell Products, R. Smith ed., (Springer, Dordrecht, The Netherlands), pp. 261-268. Read paper
6) Stern, B., Olsen, L.C., Τröβe, C., Ravneberg, H. and Pryme, I.F. (2007) Improving mammalian cell factories : The selection of signal peptide has a major impact on recombinant protein synthesis and secretion in mammalian cells. Trends Cell Mol. Biol. 2: 1-17. Read paper
7) Τröβe, C., Ravneberg, H., Stern, B. and Pryme, I.F. (2007) Vectors encoding seven oikosin signal peptides transfected into CHO cells differ greatly in mediating Gaussia luciferase and human endostatin production although mRNA levels are largely unaffected. Gene Regulation and Systems Biology 1: 303-312. Read paper
8) Stern, B., Optun, A., Ravneberg, H., Pruβ, T., Hesketh, J.E., Tauler , A. and Pryme, I.F. A signal peptide mutagenesis approach to improve the productivity of mammalian cell factories. ESACT Proceedings. Submitted.
9) Ravneberg et al. The exchange of Vargula hilgendorfii luciferase signal peptide with that from Gaussia princeps luciferase, follistatin, albumin or interleukin-2 modulates synthesis and secretion levels of Vargula luciferase in transfected CHO cells (manuscript in preparation).